The fecal coliform group is commonly used as an indicator of fecal contamination in water, which can be associated with diseases like dysentery. The test for fecal coliform bacteria involves several steps, including sample collection, processing, and analysis. Call Hambly Water Conditioning today to help collect a sample and git it to a qualified lab for the testing.
To properly collect a water sample for the fecal coliform test, it is important to follow these guidelines:
1. Choose a representative sampling location: Select a site that reflects the water quality you want to assess, typically near potential sources of contamination like sewage discharge points or areas with human or animal activities.
2. Use sterile sampling containers: Sterile bottles or vials specifically designed for microbiological testing should be used to collect the water sample. These containers are typically pre-sterilized and have a secure lid to prevent contamination.
3. Collect a sufficient volume: The amount of water needed may vary depending on the specific requirements of the test, but typically, a minimum of 100 milliliters (ml) is recommended. Ensure that the sample is taken at a consistent depth, such as elbow-deep, to minimize variations.
Once the sample is collected, it needs to be processed and analyzed in a laboratory. The test procedure may involve the following steps:
1. Filtration: The water sample is passed through a filter membrane to concentrate any bacteria present in the water.
2. Incubation: The filter membrane is placed on a selective agar medium that promotes the growth of fecal coliform bacteria. The plate is then incubated at a specific temperature, usually around 35-37°C, for a defined period, typically 24 hours.
3. Colony identification: After incubation, the colonies that appear on the agar plate are examined. Fecal coliform bacteria, such as Escherichia coli (E. coli), typically exhibit specific characteristics, such as a distinctive appearance, which helps differentiate them from other bacteria.
Microscopy can also be used to identify fecal coliform bacteria in a sample. This method involves staining the bacteria and examining them under a microscope, looking for specific morphological features that indicate the presence of fecal contamination.
Proper storage of the sample is crucial to maintain the integrity of the bacteria for testing. It is recommended to keep the sample at a cool temperature, preferably refrigerated, and analyze it as soon as possible to prevent any changes in bacterial viability.
The timeframe for the test can vary depending on the laboratory's workload and the specific testing methods used. Typically, the entire process, from sample collection to obtaining results, can take anywhere from a few days to a week.
Hambly Water Conditioning works with certified environmental labs to identify the presence of disease causing bacteria in your well water. Once presence is confirmed Hambly will properly sanitize your well, plumbing envelope and all of the fixtures in your home. The sanitation process takes 24 hours and then Hambly will install a suitable pre-filter and UV disinfection system to ensure safe drinking water for your family going forward.
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